Novel molecular test to detect Canadian toad environmental DNA

Primary Research Organization: SLR Consulting (Canada) Ltd.

Start Date: January 2024

Anticipated End Date: December 2025

Project Summary and Scope of Work 

SLR’s objectives in this project are to:
• Obtain DNA samples from a wide geographic distribution to capture the genetic diversity of Canadian toad in Alberta through
– Support from producers to provide DNA samples from salvage and translocation programs; and
– A targeted field sampling program
• Generate novel DNA sequences for Canadian toad;
• Develop a new eDNA assay for the detection of Canadian toad eDNA that is optimized for Albertan populations; and
• Validate the new eDNA assay to confirm success in real-world inventory scenarios.